Agilent Bio IEX
Agilent Bio IEX HPLC Columns
Agilent Bio Ion-Exchange HPLC columns are packed with polymeric, nonporous, ion-exchange particles. Bio Ion-Exchange Columns are designed for high resolution, high recovery and highly efficient separations of peptides, oligonucleotides and proteins. The Bio IEX family offers strong cation-exchange (SCX), weak cation-exchange (WCX), strong anion-exchange (SAX) and weak anion-exchange (WAX) phases. All Bio Ion-Exchange phases are available in 1.7, 3, 5 and 10 µm non-porous particles sizes.
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- Features
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- Highly crosslinked and rigid nonporous poly(styrene divinylbenzene) (PS/DVB) particles are grafted with a hydrophilic, polymeric layer, eliminating nonspecific binding
- Uniform, densely packed ion-exchange functional groups are chemically bonded to the hydrophilic layer (multiple ion-exchange groups per anchoring) to increase column capacity
- Particles, coating and bonding are resistant to high pressures, promoting higher resolution and faster separations
- Multiple ion-exchange groups are captured on one anchoring to increase capacity
- Specifications
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Column phase SCX (Strong cation-exchange) – SO3H
WCX (Weak cation-exchange) – COOH
SAX (Strong anion-exchange) – N(CH3)3
WAX (Weak anion-exchange) – N(C2H5)2Packing Non-porous, poly(styrene divinylbenzene) (PS/DVB), grafted hydrophilic coating and bonded with a uniform, ion-exchange layer Particle size 1.7, 3, 5 and 10 µm Pore structure Non-porous pH stability 2 - 12 Operating temperature limit 80 ºC Column hardware operating pressure limit 600 bar (8,700 psi) for stainless steel column hardware
400 bar (5,800 psi) for PEEK column hardwareParticle operating pressure limit 275 bar (4,000 psi) for 10 µm particles
413 bar (6,000 psi) for 5 µm particles
551 bar (8,000 psi) for 3 µm particles
689 bar (10,000 psi) for 1.7 µm particlesMobile phase compatibility Compatible with aqueous solution buffers, acetonitrile/acetone/methanol and water mixtures. Commonly used buffers: phosphate, tris, MES and acetate Working flow rate Typical 0.1 - 1 mL/min for a 4.6 mm id column or 2.1 mm id column; always start a low flow rate and set default to the maximum hardware and/or particle pressure, whichever is lower. Dynamic binding capacity NP3 NP5 NP10 SCX 53 mg/mL 38 mg/mL 20 mg/mL WCX 19 mg/mL 15 mg/mL 10 mg/mL SAX 35 mg/mL 28 mg/mL 17 mg/mL WAX 26 mg/mL 18 mg/mL 12 mg/mL - Literature
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Agilent Bio IEX Column Brochure
Now you have more columns and more choices to improve your charged-variant analysis of proteins with the Agilent Ion-exchange BioHPLC columns.
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Agilent Bio IEX Columns Data Sheet
Data sheet for the Agilent Bio IEX Columns
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Agilent Bio Column Selection Guide
This comprehensive guide will help you find the right column for your characterization workflow. We have also included advice and tips on method development, solvent choice, mobile phase modification, optimization, and many example separations, all to assist you in column selection and method development.
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pH Gradient Elution for Improved Separation of Monoclonal Antibody Charge Variants
In this application note, we demonstrate the benefits of using pH gradient elution for separation of charge isoforms of monoclonal antibodies using Agilent Bio MAb columns
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